Thermo Fisher Scientific Smad2 Monoclonal Antibody (9A3)
Smad2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Monoclonal Antibody (Clone 9A3). Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. 액상 형태로 제공되며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9A3 |
| Immunogen | Recombinant human Smad2 protein (N-terminal 1–200 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Target Information
SMAD2 (also known as MADH2 or MAD2) regulates multiple cellular processes such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with TGF-beta receptors through the SMAD anchor for receptor activation, and its translocation into the nucleus is central to TGF-beta signaling. Phosphorylation by ERK1 enhances SMAD2 transcriptional activity, while calmodulin negatively regulates it. Upon TGF-beta stimulation, SMAD2 is phosphorylated, dissociates from SARA, and associates with SMAD4 to form transcriptional complexes in the nucleus. SMAD2 can also mediate activin signaling via phosphorylation by activin type 1 receptor kinase. Multiple alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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