Thermo Fisher Scientific E-selectin Monoclonal Antibody (UZ5)
마우스 E-selectin을 검출하는 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합합니다. Rat IgG2b 형식의 비결합 항체이며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
| Flow Cytometry (Flow) | 1:100–1:200 |
| Immunoprecipitation (IP) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UZ5 |
| Immunogen | LPS-activated mlEND1 cells expressing E-selectin at their cell surface |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_558253 |
Product Specific Information
MA1-06505 detects E-selectin in mouse samples.
It has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, FACS, ICC, and IF applications.
The immunogen is LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Target Information
Leukocytes adhere to blood vessel endothelium during extravasation in postcapillary venules of lymph nodes and in inflamed tissues. This process involves a cascade of adhesion receptors, including selectins and members of the immunoglobulin superfamily.
E-selectin (CD62E) is an endothelial cell-specific selectin expressed only after activation by proinflammatory cytokines such as IL-1, TNFα, and bacterial lipopolysaccharides via NFκB-dependent signaling.
E-selectin is associated with endothelial cells in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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