Thermo Fisher Scientific TOP2A Monoclonal Antibody (OTI3E11), TrueMAB
Human TOP2A 단백질(aa 1100–1531)에 특이적인 Mouse IgG1 단클론 항체로, Western blot 및 IHC(파라핀) 적용 가능. Lyophilized 형태로 제공되며 PBS buffer(8% trehalose) 기반. 연구용으로 DNA 합성 및 세포분열 관련 TOP2A 검출에 활용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1100–1531 of human TOP2A produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Topo II alpha plays critical roles in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker for late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also implicated in tumor cell drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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