Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor 660, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.25 µg/test

Species Reactivity

• Human
• Mouse
• Rhesus monkey

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eBio4B10 (4B10) monoclonal antibody reacts with mouse and human T-bet, a Th1-specific T-box transcription factor critical to Th1 CD4+ T cell lineage development.
T-Bet expression is induced by IFN-γ and regulates IFN-γ, IL-12Rβ, and IL-2 expression through chromatin remodeling. It also plays a role in class-switch recombination in B-cells.

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Validated using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤0.25 µg/test in 100 µL final volume. Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Alexa Fluor 660 emits at 690 nm and is ideal for spectral cytometers; compatible with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm
Emission: 690 nm
Laser: Red Laser

Target Information

T-bet (TBX21) is a T-cell specific transcription factor containing a T-box DNA-binding domain. It regulates interferon-gamma expression and directs Th1 lineage differentiation.
T-bet cooperates with Stat4 in chromatin remodeling for Th1 gene expression. Genetic variations are linked to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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