Thermo Fisher Scientific RON Polyclonal Antibody
Rabbit polyclonal antibody targeting human CD136 (RON/MST1R) for IHC-P applications. Recognizes receptor tyrosine kinase involved in cell proliferation and motility. Recommended positive control: colon surface epithelium. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide: internal domain of human CD136, conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2251097 |
Product Specific Information
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for staining paraffin sections.
A suggested positive control for this product is colon surface epithelium.
Rabbit anti-Human CD136 antibody detects CD136, a receptor tyrosine kinase also known as Macrophage Stimulating 1 Receptor (MST1R) and RON (Recepteur d’origine nantais).
Target Information
MST1R (RON) is a receptor tyrosine kinase involved in cell proliferation and motility. The protein is a membrane-spanning, disulfide-linked heterodimer resulting from cleavage of a glycosylated precursor into 35-kDa (alpha) and 150-kDa (beta) subunits. Ligand binding results in tyrosine phosphorylation of the beta chain.
In knockout studies, MST1R/RON (-/-) mice failed to survive past the peri-implantation period. The MST1R/RON gene has been mapped to chromosome 3p21, a region frequently deleted or mutated in small cell lung and renal carcinoma, and implicated in the progression of several epithelial cancers.
MST1R/RON expression has been documented in many normal human tissues. ESTs have been isolated from tissue libraries including normal colon, mouth, prostate, and testis, as well as cancerous colon, prostate, stomach, and uterus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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