Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: Assay-Dependent

Control (Ctrl)

• Tested Dilution: Assay-Dependent

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–786 series
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Functional Grade
• Pacific Orange
• Custom conjugation available

Product Specific Information

The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.

• Applications Reported: APC-eFluor® 780 Rat IgG2a Isotype Control (APC-Alexa Fluor® 750 replacement) is reported for use in flow cytometry.
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Use at the same concentration as the experimental antibody.
• Excitation/Emission: 633–647 nm excitation; 780 nm emission (Red laser).
• Light Sensitivity: Sensitive to photo-induced oxidation; protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rat IgG2a is an immunoglobulin subclass commonly used in research and diagnostics. It activates complement and mediates ADCC, with a molecular weight of approximately 160 kDa. Composed of two heavy and two light chains, it serves as a control or reference antibody in rat model experiments, including flow cytometry and immunohistochemistry.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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