Thermo Fisher Scientific NF2 Polyclonal Antibody
NF2 단백질(Merlin)을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ELISA, IP 등에 적합합니다. Human, Mouse, Rat 시료에서 반응하며, Affinity Chromatography로 정제된 고순도 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 477–576 of human NF2 (NP_000259.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.66 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913625 |
Product Specific Information
Positive test controls: A549, A431, HeLa, SKOV3, Mouse kidney, Mouse heart, Rat brain.
Subcellular localization: Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Peripheral membrane protein, Cytoskeleton, Filopodium membrane, Perinuclear region, Ruffle membrane.
Immunogen sequence:
TKPTYPPMNP IPAPLPPDIP SFNLIGDSLS FDFKDTDMKR LSMEIEKEKV EYMEKSKHLQ EQLNELKTEI EALKLKERET ALDILHNENS DRGGSSKHNT
Target Information
NF2 (Merlin) encodes a protein similar to members of the ERM family that link cytoskeletal components with membrane proteins. Merlin interacts with cell-surface and cytoskeletal regulatory proteins, and is expressed highly during embryonic development. In adults, expression is notable in Schwann cells, meningeal cells, lens, and nerve.
Mutations in NF2 are associated with neurofibromatosis type II, characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and several minor isoforms are generated by alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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