Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (6F10), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6F10

Immunogen

Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• APC
• PE
• PerCP
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932240

Product Specific Information

A 1:1,000 dilution of MA5-45786 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Target Information

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an alpha,beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.