Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (6F10), PE
4-Hydroxy-2-hexenal 및 4-Hydroxynonenal 변형 단백질 검출용 단일클론 항체. PE 형광 표지로 유세포분석, ELISA, WB, ICC에 적합. Mouse IgG2b 아이소타입. 565/576 nm 형광 특성. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6F10 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932242 |
Available Formats:
- Unconjugated (MA5-27556)
- APC (MA5-45785)
- FITC (MA5-45786)
- PerCP (MA5-45787)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45788 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins.
Does not detect free 4-HHE or 4-HNE.
Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha,beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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