Thermo Fisher Scientific 4-Hydroxynonenal Monoclonal Antibody (12F7), APC
4-Hydroxynonenal(4-HNE) 변형 단백질을 특이적으로 검출하는 APC 결합 마우스 단클론 항체. Western blot, ICC/IF, ELISA 등 다양한 연구용 응용 가능. 보존제 무첨가, 4°C 보관, Protein G 정제. 진단용 사용 불가.
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Thermo Fisher Scientific 4-Hydroxynonenal Monoclonal Antibody (12F7), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12F7 |
| Immunogen | Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932243 |
Additional Formats
- Unconjugated (MA5-27570)
- FITC (MA5-45790)
- PE (MA5-45792)
- PerCP (MA5-45791)
- Request custom conjugation available
Product Specific Information
A 1:1,000 dilution of MA5-45789 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxynonenal (4-HNE) modified proteins.
Does not detect free 4-Hydroxynonenal.
Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, 4-hydroxy-2-nonenal) is an alpha, beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells.
It is the primary alpha, beta-unsaturated hydroxyalkenal formed during this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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