Thermo Fisher Scientific Alpaca anti-Mouse IgG2b Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor 647

Applications

Tested Dilution

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:1,000

Dot blot (DB)

Assay-dependent

Immunomicroscopy (IM)

1:1,000

Product Specifications

Host/Isotype

Alpaca / IgG2b

Expression System

E. coli

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

CTK0105/CTK0106

Immunogen

Purified mouse IgG2b

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2868385

Target

IgG2b

Antibody Form

VHH

Product Specific Information

Biclonal nanobodies specific for mouse IgG2b Fc. CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM

Non Cross-Reactivity includes: Rabbit, rat, goat, sheep, guinea pig, human, and macaque serum and to mouse IgG1, IgG2a, IgG2c, and IgG3.

Rapid workflow: Simultaneous incubation of mouse IgG2b primary antibody and Alpaca anti-mouse IgG2b Nano (VHH) recombinant secondary antibody.

Offered through a partnership with ChromoTek

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.