Thermo Fisher Scientific C-Jun Monoclonal Antibody (OTI5E9), TrueMAB
인간 c-Jun 단백질을 인식하는 마우스 단클론 항체. Western blot에 적합하며, carrier-free 형태로 제공되어 결합 실험에 활용 가능. 동결건조 상태로 제공되며, PBS 완충액과 trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
- Application: Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E9 |
| Immunogen | Full length human recombinant protein of human JUN produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
c-Jun is a transcription factor that binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
It promotes NR5A1 activity when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP pathway stimulation.
It also plays a role in KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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