Thermo Fisher Scientific ATF4 Polyclonal Antibody
ATF4 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보관하며 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| ChIP assay (ChIP) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 146–333 of Human ATF4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.7 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545052 |
Product Specific Information
- Recommended positive controls: HeLa (4 µg/mL tunicamycin treatment for 16 hr)
- Predicted reactivity: Mouse (83%), Rat (85%), Pig (89%), Sheep (86%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a transcription factor originally identified as a mammalian DNA-binding protein that binds a tax-responsive enhancer element in the LTR of HTLV-1. It is also known as cAMP-response element binding protein 2 (CREB-2).
The encoded protein belongs to a family of DNA-binding proteins including AP-1, CREBs, and CREB-like proteins, sharing a leucine zipper region for protein-protein interactions and a basic domain for DNA binding.
Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 within a large inverted duplication region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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