Thermo Fisher Scientific PHGDH Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 209 and 437 of Human PHGDH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PHGDH,uniProtId:O43175-1,ncbiNodeId:9606,antigenRange:209-437,antigenLength:533,antigenImageFileName:PA5-27579_PHGDH_O43175-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27579_PHGDH_O43175-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545055

Product Specific Information

Recommended positive controls: 293T, A431, Jurkat, Raji, HeLa.

Predicted reactivity: Mouse (96%), Rat (97%), Pig (90%), Chimpanzee (100%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.