Thermo Fisher Scientific PHGDH Polyclonal Antibody
Thermo Fisher Scientific의 PHGDH Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 Western blot, IHC, ICC 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 항체로, PHGDH 단백질 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–175 of Human PHGDH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.87 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545054 |
Product Specific Information
- Recommended positive controls: HeLa, 293T, A431, HeLaS3, mouse brain, SK-N-DZ
- Predicted reactivity: Mouse (99%), Rat (97%), Pig (97%), Chimpanzee (100%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The PHGDH gene encodes an enzyme involved in the early steps of L-serine synthesis in animal cells.
L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity.
Mutations in this gene have been associated with congenital microcephaly, psychomotor retardation, and other symptoms.
Multiple alternatively spliced transcript variants have been identified, though the full-length nature of most is not known.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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