Thermo Fisher Scientific CD20 Monoclonal Antibody (HI47), Pacific Orange

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

HI47

Immunogen

CD20 antigen

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

View additional formats

• Pacific Blue
• PE
• PE-Cyanine5.5
• PE-Texas Red
• PerCP
• Request custom conjugation

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10375578

Product Specific Information

Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Target Information

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

Analyte Specific Reagent