Thermo Fisher Scientific beta Actin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 42 publications 42 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2000-1:10000

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Immunoprecipitation (IP)

Assay-Dependent

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Dot blot (DB)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate

Published species

Arthropod, Bison, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Actin,uniProtId:P60709-1,ncbiNodeId:9606,antigenRange:1,antigenLength:375,antigenImageFileName:PA1-16889_beta_Actin_P60709-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16889_beta_Actin_P60709-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7-8

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568434

Product Specific Information

Suggested positive control: Hela whole cell extract.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.