Thermo Fisher Scientific beta Actin Polyclonal Antibody
Thermo Fisher Scientific의 beta Actin Polyclonal Antibody는 다양한 종에서 반응하는 비결합형 항체로, Western blot, IHC, ICC 등 다양한 실험에 사용됩니다. 세포골격 연구 및 단백질 로딩 컨트롤에 적합하며, 높은 특이성과 재현성을 제공합니다.
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- PA116889
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | View 42 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | – |
| Immunoprecipitation (IP) | Assay-Dependent | View 2 publications |
| ChIP assay (ChIP) | – | View 1 publication |
| Dot blot (DB) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate |
| Published Species | Arthropod, Bison, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-terminus of human beta actin (Swiss-prot entry P60709); highly conserved with gamma actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7–8 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568434 |
Product Specific Information
Suggested positive control: HeLa whole cell extract.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein involved in cell motility, structure, and integrity. Beta and gamma actin coexist in most cell types as cytoskeletal components. Beta actin is ubiquitously expressed in all eukaryotic cells and often used as a loading control in protein detection assays such as Western blotting. Antibodies to beta actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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