Thermo Fisher Scientific Claudin 1 Polyclonal Antibody
Claudin-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 적합. Human, Mouse, Dog 등 반응성 보유. 항원 친화 크로마토그래피로 정제되었으며 PBS/BSA 버퍼에 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Claudin 1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:200 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human claudin-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2244877 |
Product Specific Information
This antibody is predicted to react with bovine, canine, porcine, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
Suggested positive controls include skin, breast carcinoma, or ovarian carcinoma.
Target Information
Claudin-1 is a transmembrane protein located in cell-cell tight junctions and acts as a co-receptor for HCV entry into hepatic cells.
Claudins are abundant in luminal epithelial sheets, maintaining epithelial cell polarity.
Claudin-1 is expressed in tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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