Thermo Fisher Scientific OPRK1 Polyclonal Antibody
Rabbit polyclonal antibody against human OPRK1 (Kappa opioid receptor). Validated for WB and IHC (Paraffin). Detects glycosylated receptor (~60 kDa). Suitable for research on pain modulation and opioid receptor signaling.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated Cys-RVRNTVQDPAYLRDIDGMNKPV (OPRK1, UniProt ID: P41145-1, antigen range 359–380) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2540489 |
Product Specific Information
- IHC (P) analysis shows positive staining of KOR in human brain tissue.
- WB analysis detects KOR in glycoprotein-enriched fractions from mock-transfected or KOR-overexpressing 293 cells.
- Although the theoretical molecular weight (MW) of KOR is ~43 kDa, the observed band in WB runs at ~60 kDa due to glycosylation.
Target Information
The OPRK1 gene encodes an opioid receptor belonging to the seven transmembrane-spanning G protein-coupled receptor family. It acts as a receptor for both endogenous ligands and synthetic opioids, leading to inhibition of adenylate cyclase activity and neurotransmitter release. This receptor is involved in pain perception and mediates the hypolocomotor, analgesic, and aversive effects of synthetic opioids. Variants in this gene are associated with alcohol dependence and opiate addiction. Alternative splicing and translational readthrough events produce multiple isoforms, including a C-terminally extended form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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