Thermo Fisher Scientific CABIN1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

-

ELISA (ELISA)

1:15,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acid residues 1881-2173 of rat cain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CABIN1,uniProtId:O88480-1,ncbiNodeId:10114,antigenRange:1881-2173,antigenLength:2182,antigenImageFileName:PA3-026_CABIN1_O88480-1_Rabbit.svg,antigenImageFileNamePDP:PA3-026_CABIN1_O88480-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2157204

Product Specific Information

PA3-026 detects calcineurin inhibitor (cain) from human and rat tissues.

PA3-026 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~240 kDa protein representing cain from rat liver extract. Immunoprecipitation of cain from rat brain tissues shows an interaction between cain and amphiphysin 1, adaptin, and dynamin 1.

The PA3-026 immunizing peptide is a His-tagged fusion protein corresponding to amino acid residues 1881-2173 from rat cain.

Target Information

Calcineurin binding protein (Cabin-1) and the corresponding rat homolog, designated Cain, are widely expressed nuclear phosphoproteins that regulate the serine/threonine phosphotase activity of calcineurin and influence T cell signaling and apoptosis. Calcineurin is required for the transcriptional activation of cytokines and the activation of various transcription factors, including NFAT, NFκB and AP-1, involved in T cell receptor (TCR)-mediated signaling. The regulation of calcineurin depends on the changes in intracellular calcium concentrations and the activity of protein kinase C. TCR activation results in PKC inducing the hyperphosphorylation of Cabin-1, which facilitates the high affinity binding of Cabin-1 to calcineurin. This complex formation, in turn, inhibits calcineurin activity and attenuates TCR-mediated signaling. Cabin-1 also associates directly with MEF-2 proteins, a family of transcription factors that regulate apoptosis signaling in T cells. This association between Cabin-1 and MEF-2 leads to the inhibition of MEF-2-mediated gene transcription and the inhibition of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.