Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (MH99), Alexa Fluor 488, eBioscience
인간 EpCAM(CD326)을 인식하는 MH99 클론의 Alexa Fluor 488 결합 단일클론 항체. Flow cytometry에 최적화되어 있으며 A549 세포 분석에 검증됨. 499/520 nm 형광 특성, 4°C 암저장, Affinity chromatography로 정제됨.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (MH99), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MH99 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11220074 |
Product Specific Information
The monoclonal antibody MH99 recognizes human CD326, also known as EpCAM (Epithelial cell adhesion molecule). This 40 kDa type I transmembrane protein is involved in calcium-independent cell-cell interactions and is expressed primarily on the basolateral surface of most epithelia.
Normal epithelia express low levels of CD326, but increased expression correlates with proliferation and progression to a mesenchymal phenotype. CD326 serves as a diagnostic marker for circulating metastatic carcinoma cells, while non-epithelial cancer cells do not express EpCAM.
Based on crossblocking studies, MH99 and 1B7 recognize overlapping epitopes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of A549 cell line. Recommended usage is 5 µL (0.5 µg) per test (100 µL final volume).
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelium. It functions as a homotypic calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing c-myc, cyclin A, and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is found on basolateral surfaces of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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