Thermo Fisher Scientific Adiponectin Monoclonal Antibody (OTI2E2), TrueMAB
Adiponectin 단백질을 인식하는 인간 반응성 마우스 단클론 항체. Western blot과 IHC(파라핀) 분석에 적합. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. 지방대사 및 인슐린 신호 조절 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 19–244 of human ADIPOQ produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates lipid and glucose metabolism. It plays a role in physiological processes such as homeostasis and obesity.
Decreased adiponectin levels are associated with obesity, insulin resistance, and type 2 diabetes. In mice, adiponectin improves insulin signaling and reduces insulin resistance by activating AMP kinase and PPAR-alpha, enhancing fatty acid transport and oxidation in skeletal muscle, and lowering intramyocellular lipid content.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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