Thermo Fisher Scientific ERK1 C-Term Polyclonal Antibody, FITC
ERK1 단백질의 C-말단을 인식하는 FITC 결합 폴리클로날 항체로, Western blot과 ELISA에 적합합니다. 인간, 마우스, 랫트에 반응하며 ERK2와는 교차 반응하지 않습니다. 형광 검출이 가능하며 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This fluorescein-conjugated, affinity-purified antibody is directed against human ERK1 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3 within the MAPK pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells. It is activated by various stimuli such as growth factors, cytokines, viral infection, and GPCR ligands. Functionally, ERK1 participates in signaling cascades that regulate proliferation, differentiation, and cell cycle progression. Upon activation by upstream kinases, ERK1 translocates to the nucleus to phosphorylate nuclear targets. Multiple alternatively spliced transcript variants encoding different ERK1 isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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