Thermo Fisher Scientific MEK1 C-Term Polyclonal Antibody, FITC
FITC 형광 표지된 MEK1 C-Term 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC, ELISA에 사용 가능하며, 고순도 친화 크로마토그래피 정제 제품입니다. 연구용으로 세포 신호전달 및 MAPK 경로 분석에 적합합니다.
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Thermo Fisher Scientific MEK1 C-Term Polyclonal Antibody, FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | 1:100 |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; -20°C long term; avoid freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This fluorescein-conjugated, affinity-purified antibody targets human MEK1 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in MAPK cascades, controlling cell growth and differentiation. It is activated by growth factors, cytokines, membrane depolarization, and calcium influx. MEK1 activation occurs via phosphorylation at serine 218 and 222 by Raf1. It plays roles in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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