ELK Biotechnology BID (p11, Cleaved-Ser100) rabbit pAb

ELK Biotechnology BID (p11, Cleaved-Ser100) rabbit pAb

제품 정보

세포 내 위치 (Cellular Localization)

• Cytoplasm, Mitochondrion membrane, Mitochondrion outer membrane
• When uncleaved, predominantly cytoplasmic
• Cleaved forms (p15, p13, p11) are associated with mitochondrial membrane
• Isoform 1, 3: Cytoplasm
• Isoform 2: Mitochondrion membrane, may be constitutively cleaved

Background

• Domain: Intact BH3 motif is required for pro-apoptotic activity and interaction with Bcl-2 family proteins (BIK, BID, BAK, BAD, BAX).
• Function: The major proteolytic product p15 BID promotes cytochrome c release. Isoforms 1, 2, and 4 induce apoptosis, while isoform 3 does not.
• Post-translational modification (PTM): Phosphorylated upon DNA damage (likely by ATM or ATR). TNF-α induces caspase-mediated cleavage of p22 BID into p15, p13, and p11 products.
• Subunit: Forms heterodimers with pro-apoptotic BAX or anti-apoptotic Bcl-2.
• Tissue Specificity:
  • Isoforms 2 and 3: spleen, bone marrow, cerebral and cerebellar cortex
  • Isoform 2: spleen, pancreas, placenta
  • Isoform 3: lung, pancreas, spleen
  • Isoform 4: lung, pancreas