Thermo Fisher Scientific CTLA-4 Monoclonal Antibody (MCT.E9)

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.01-0.1 µg/mL

-

Blocking Assay (BLOCK)

10-50 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MCT.E9

Immunogen

Recombinant mouse CTLA-4-Fc

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Cytotoxic T-lymphocyte-associated protein 4 (CTLA4), also known as CD152, is one of the first well studied immune checkpoint molecules. CTLA4 is expressed on T cells and its expression level can be up-regulated after T cell activation. CTLA4 has two ligands CD80 (B7-1) and CD86 (B7-2). CTLA4 can compete with CD28 binding with these two ligands and transmits an inhibitory signal to T cells. Therapeutic antibodies against CTLA4 can help to stimulate T cells which can then target many cancer cells. Mouse CTLA-4 antibodies with has blocking effect against CD80/CD86 binding with CTLA4 can serve as a great surrogate antibody in mouse model.
GenScript Mouse CTLA-4 Antibody (MCT.E9), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant mouse CTLA4-Fc

Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.