Thermo Fisher Scientific HTRA2 Polyclonal Antibody
HTRA2 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 인간 시료 반응성, 액상 형태, 1 mg/mL 농도. 세포자멸사 관련 연구용으로 적합하며, U937 cell lysate를 양성 대조로 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (IHC) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 16 amino acids near the C-terminus of human Omi |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152521 |
Product Specific Information
- Suggested positive control: U937 cell lysate
- PA5-20010 can be used with blocking peptide PEP-0130
Target Information
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. A serine protease, Omi/HtrA2, is released from mitochondria upon induction of apoptosis. This mitochondrial protein binds to IAP and contains a conserved IAP-binding motif (AVPS) at its N terminus. Deregulated expression of Omi in the cytoplasm induces apoptosis, suggesting its role in mitochondrial apoptotic pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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