Thermo Fisher Scientific PUMA alpha Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The PA5-20008 immunogen is located within the first 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA.
In Western blot applications, this antibody detects a band at approximately 23 kDa. A suggested positive control is K562 cell lysate.
PA5-20008 can be used with blocking peptide PEP-0128.
Predicted species reactivity based on immunogen sequence: Rat (79%), Mouse (79%).

Target Information

PUMA is a pro-apoptotic Bcl-2 family member, including Bax and Noxa, which are transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins, PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53 activation.
PUMA may act as a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduces the apoptotic response to p53, suggesting PUMA’s role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.