Thermo Fisher Scientific PUMA alpha Polyclonal Antibody
PUMA 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간 및 랫드 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 세포사멸 연구 및 p53 경로 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 10–20 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Rat, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 14 amino acid peptide near the amino terminus of human PUMA isoform 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156638 |
Product Specific Information
The PA5-20008 immunogen is located within the first 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA.
In Western blot applications, this antibody detects a band at approximately 23 kDa. A suggested positive control is K562 cell lysate.
PA5-20008 can be used with blocking peptide PEP-0128.
Predicted species reactivity based on immunogen sequence: Rat (79%), Mouse (79%).
Target Information
PUMA is a pro-apoptotic Bcl-2 family member, including Bax and Noxa, which are transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins, PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53 activation.
PUMA may act as a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduces the apoptotic response to p53, suggesting PUMA’s role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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