Thermo Fisher Scientific ARFGAP1 Monoclonal Antibody (OTI1F6), TrueMAB
ARFGAP1 단백질을 인식하는 Mouse IgG2b 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제. 동결건조 형태로 안정적 보관 가능.
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Thermo Fisher Scientific ARFGAP1 Monoclonal Antibody (OTI1F6), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F6 |
| Immunogen | Full length human recombinant protein of ARFGAP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ArfGAP1 is a 406-amino-acid regulatory protein containing an Arf-GAP domain that activates the GTPase activity of ADP ribosylation factor (ARF).
It promotes hydrolysis of ARF1-bound GTP and facilitates dissociation of coat proteins from Golgi-derived membranes and vesicles, playing a key role in membrane trafficking and vesicle transport.
Its activity is stimulated by phosphoinositides and inhibited by phosphatidylcholine.
Two transcript variants encoding different isoforms have been reported, with ubiquitous expression in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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