Thermo Fisher Scientific PRKCE Monoclonal Antibody (OTI4D12), TrueMAB
인간 PRKCE 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, ICC/IF, Flow Cytometry에 적합합니다. Lyophilized 형태이며 PBS buffer에 보존, 연구용으로 신호전달 관련 PKC epsilon 분석에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D12 |
| Immunogen | Full length human recombinant protein of human PRKCE produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated by intracellular signal transduction pathways. In humans, at least 12 PKC isoforms have been identified, differing in structure, distribution, and function.
PKC alpha, beta I, beta II, and gamma form the conventional family, dependent on Ca²⁺ and phospholipids. PKC family members phosphorylate diverse protein targets, participate in various signaling pathways, and act as receptors for phorbol esters, which are tumor promoters.
Each PKC isoform exhibits unique expression and plays distinct cellular roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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