Thermo Fisher Scientific Phospho-AKT2 (Ser474) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within human AKT2 aa 450-481(phospho S474) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT2,uniProtId:P31751-1,ncbiNodeId:9606,antigenRange:450-481,antigenLength:481,antigenImageFileName:PA5-119813_AKT2_P31751-1_Rabbit.svg,antigenImageFileNamePDP:PA5-119813_AKT2_P31751-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2913385

Product Specific Information

Positive Control: Hela treated with Calyculin A whole cell lysate, Hela treated with insulin whole cell lysate, human kidney tissue, mouse testis tissue.

Subcellular Location: Cell membrane, Nucleus, Early endosome, Cytoplasm.

Target Information

AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.