Thermo Fisher Scientific Human FGF-10 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-dependent

-

In vitro Assay (IV)

-

View 152 publications 152 publications

Miscellaneous PubMed (Misc)

-

View 38 publications 38 publications

Product Specifications

Species

Human

Published species

Bovine, Cat, Chicken, Dog, Human, Mouse, Pig, Rat, Reptile

Expression System

E. coli

Amino acid sequence

MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

Molecular weight

19.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Assay 1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg. Assay 2: Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-15.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

100-00CC-500UG will be provided as 2 x 250 µg (100-00CC-250UG).

Recombinant Human FGF-10 is a 19.3 kDa protein consisting of 170 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.