Thermo Fisher Scientific ARC Polyclonal Antibody

Thermo Fisher Scientific ARC Polyclonal Antibody

Applications and Tested Dilutions

• Western Blot (WB): 1:500–1:1,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:100

Product Specifications

Target Information

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms.
Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death.

ARC inhibits extrinsic apoptotic pathways in two different ways:

1. By interacting with FAS and FADD upon FAS activation, blocking death-inducing signaling complex (DISC) assembly.
2. By interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting soluble CASP8 available for DISC-mediated activation.

ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX, resulting in BAX inactivation and prevention of mitochondrial dysfunction and release of pro-apoptotic factors.

Mutations affecting the gene can result in myoclonus, familial cortical disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.