Thermo Fisher Scientific Cyclin B2 Monoclonal Antibody (X29.2)
Cyclin B2 단백질 검출용 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 사람, 생쥐, 영장류, Xenopus 반응성. Golgi 영역 특이적 염색 및 Cyclin B1 교차 반응 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | X29.2 |
| Immunogen | Xenopus laevis cyclin B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536864 |
Product Specific Information
MA1-156 detects Cyclin B2 in mammalian samples and has been successfully used in Immunofluorescence, Immunoprecipitation, IHC (P), and Western Blot procedures.
In Immunoprecipitation and Western Blot analysis, MA1-156 shows a prominent band at approximately 51 kDa in camptothecin and hydroxyurea-treated cells, and an additional unknown band around 80 kDa.
In Immunofluorescence applications, MA1-156 shows Cyclin B2 staining consistent with the Golgi region, whereas MA1-155 shows Cyclin B1 co-localization with microtubules.
MA1-156 reacts with Cyclin B2 from Xenopus laevis and mammalian sources. In Western blot applications, X29.2 also cross-reacts with Cyclin B1.
Target Information
Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery.
B1 and B2 differ in their subcellular localization: Cyclin B1 co-localizes with microtubules, whereas Cyclin B2 is primarily associated with the Golgi region.
Cyclin B2 also binds to transforming growth factor beta RII and may play a key role in transforming growth factor beta-mediated cell cycle control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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