Thermo Fisher Scientific PKC alpha Monoclonal Antibody (MC5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

5 µg

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

MC5

Immunogen

Purified bovine brain Protein Kinase C alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC alpha,uniProtId:P04409-1,ncbiNodeId:9913,antigenRange:1-672,antigenLength:672,antigenImageFileName:MA1-157_PKC_alpha_P04409-1_House_mouse.svg,antigenImageFileNamePDP:MA1-157_PKC_alpha_P04409-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536865

Product Specific Information

Western blot analysis of MA1-157 detects an ~80 kDa protein in human, bovine, non-human primate, mouse, rat, dog and rabbit cells. In Jurkat, rat brain and MDCK cell lysates, a weak unknown band at ~140 kDa is also detected. MA1-157 shows specificity to PKC alpha and is non-reactive to other PKC family members.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.