Thermo Fisher Scientific Androgen Receptor Monoclonal Antibody (AN1-15)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Gel Shift (GS)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rat / IgG

Class

Monoclonal

Type

Antibody

Clone

AN1-15

Immunogen

A fusion protein corresponding to residues 331-572 of human androgen receptor. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Androgen Receptor,uniProtId:P10275-1,ncbiNodeId:9606,antigenRange:331-572,antigenLength:920,antigenImageFileName:MA1-150_Androgen_Receptor_P10275-1_Rat.svg,antigenImageFileNamePDP:MA1-150_Androgen_Receptor_P10275-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325419

Product Specific Information

MA1-150 detects androgen receptor from human, Rhesus monkey, rat and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors.

MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining.

The MA1-150 immunogen is a fusion protein corresponding to residues 331-572 of human androgen receptor.

Reconstitute with PBS.

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy`s disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.