Thermo Fisher Scientific Androgen Receptor Monoclonal Antibody (AN1-15)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-150 detects androgen receptor from human, rhesus monkey, rat, and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors.

MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures.
By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues.
Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining.

The MA1-150 immunogen is a fusion protein corresponding to residues 331–572 of human androgen receptor.

Reconstitution: Reconstitute with PBS.

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor (~90 kDa) has three major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain.

The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon ligand binding, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and stimulates transcription of androgen-responsive genes.

AR signaling is essential for development and function of male reproductive organs (prostate, epididymis) and also plays roles in muscle, hair follicles, and brain.
Androgen receptor is a phosphoprotein that regulates MAP kinase signaling; inhibition of MEK1/2 correlates with AR phosphorylation changes.

Abnormalities in AR signaling are linked to diseases such as prostate cancer, Kennedy’s disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.