Thermo Fisher Scientific SUMO3 Polyclonal Antibody
Rabbit polyclonal antibody against human SUMO3 protein for WB, ICC/IF, and ELISA applications. Lyophilized form, unconjugated, purified by ion-exchange chromatography. Provides high specificity for SUMOylation-related research in neurodegenerative and ...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:4,000–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human SUMO-3 protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 5 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Target Information
Small ubiquitin-related modifier proteins (SUMO) are members of the ubiquitin-like protein family. SUMO-3 regulates amyloid beta generation and may be critical in the onset or progression of Alzheimer’s disease. SUMO-2/3 forms poly-SUMO chains. SUMO-1, SUMO-2, and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies, and cytoplasm, respectively. SUMOylation is essential for mammalian cell cycle regulation; SUMO-2/3 localizes to centromeres and condensed chromosomes where it binds CENP-E.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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