Thermo Fisher Scientific Apg8 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Anti-Apg8 antibody (also known as Atg8) is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Target Information

Ubiquitin-like proteins fall into two classes:

1. Ubiquitin-like modifiers (UBLs) — function as modifiers similar to ubiquitin. Examples: SUMO, Rub1 (Nedd8), Apg8, Apg12.
2. Ubiquitin-domain proteins (UDPs) — include parkin, RAD23, and DSK2, containing domains related to ubiquitin but not conjugated to other proteins.

Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast.
During starvation, yeast cells engulf their own cytoplasm into vacuoles via autophagic bodies, forming double-membraned autophagosomes that fuse with vacuoles/lysosomes.
This process is conserved in mammals. Two gene sets, APG and AUT, are involved, responsible for two ubiquitin-like systems: Apg12 and Apg8.
Apg12 is synthesized in its mature form and conjugates primarily with Apg5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.