Thermo Fisher Scientific Phospho-p53 (Ser46) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• It is not recommended to aliquot this antibody.
• This antibody is not cross-reactive with p53 phosphorylated at other sites.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation.
p53 up-regulation leads to activation of specific genes such as p21waf1, resulting in G1 or G2/M arrest.
It consists of several domains: N-terminal transactivation, central DNA-binding, oligomerization, and C-terminal regulatory domains.

Phosphorylation at various sites regulates p53 activity:

• Ser15 phosphorylation is critical for activation and stabilization.
• Ser392 phosphorylation is associated with tumor formation.

p53 plays a key role in blocking proliferation of damaged cells and acts as a natural anticancer factor. It is also linked to aging, oxidative stress, and cognitive function via long-term potentiation (LTP).
While expressed at low levels in normal cells, p53 is highly expressed in many transformed cell lines, contributing to malignancy.
Mutations in the TP53 gene can lead to loss of tumor suppressor activity and are found in various cancers, including hereditary cancer syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.