Thermo Fisher Scientific CD31/PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (PECAM1/3539)
인간 CD31/PECAM-1 단백질을 타겟으로 하는 마우스 단일클론 항체로, 내피세포 마커로 사용됩니다. Western blot 및 면역조직화학(IHC)에 적합하며, 액상 형태로 제공됩니다. 단백질 A/G 정제, 4°C 보관 조건.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PECAM1/3539 |
| Immunogen | Recombinant fragment (around aa 625–738) of human CD31 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling through immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Upon kinase-mediated phosphorylation, these motifs provide docking sites for protein-tyrosine phosphatases.
CD31 is expressed throughout the vascular system, mainly at junctions between adjacent endothelial cells. Its N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays diverse roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, macrophage phagocytosis, IgE-mediated anaphylaxis, and thrombosis, making it a key regulatory molecule in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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