Thermo Fisher Scientific ATG4C Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:5000

-

Immunohistochemistry (IHC)

1:200-1:5000

-

Product Specifications

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide as a part of human, rat, mouse human, rat, mouse cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3) conjugated to immunogenic carrier protein has been used as the immunogen.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2227751

Product Specific Information

Reconstitute with 150 µL of distilled water.

Centrifuge to remove any insoluble material.

Target Information

ATG4C is involved in autophagy, the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.