Thermo Fisher Scientific Phospho-(Ser/Thr) ATM + ATR Substrate Monoclonal Antibody (G.770.10)

Thermo Fisher Scientific Phospho-(Ser/Thr) ATM + ATR Substrate Monoclonal Antibody (G.770.10)

Applications

• Western Blot (WB)

Tested Dilution: 1:1,000
Publications: - [References not provided]

Product Specifications

Product Specific Information

• It is not recommended to aliquot this antibody.
• This antibody is not cross-reactive with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs.
• This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position.

Target Information

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair.
The identified substrates for ATM include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1.
The essential requirement for the substrates of ATM/ATR is S/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases.
Positively charged residues surrounding the S/T*Q are negative determinants for substrate phosphorylation.
The complex phenotype of AT cells suggests that it likely has additional substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.