Thermo Fisher Scientific Phospho-(Ser/Thr) ATM + ATR Substrate Monoclonal Antibody (G.770.10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

All

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.770.10

Immunogen

Synthetic phospho-ATM/ATR substrate peptides

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

192 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10978748

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs.

This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position.

Target Information

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation. The complex phenotype of AT cells suggests that it likely has additional substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.