Thermo Fisher Scientific ATP6V1B1 Monoclonal Antibody (OTI3B11), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Vacuolar-type H⁺-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. It pumps protons against an electrochemical gradient, while F-ATPases reverse this process to synthesize ATP.
V-ATPase comprises a peripheral V1 domain (ATP hydrolysis) and an integral V0 domain (proton translocation). The V1 domain includes nine subunits (A–H), and the V0 domain includes five (a, d, c, c’, c”).
The V1 B subunit exists as two isoforms:

• B1 isoform: Functions in proton secretion in the inner ear, maintaining endolymph pH and normal auditory function. Mutations cause distal renal tubular acidosis with sensorineural deafness.
• B2 isoform: Expressed in kidney and osteoclasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.