Thermo Fisher Scientific POLR2E Monoclonal Antibody (OTI3B5), TrueMAB
POLR2E 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. Dog, Human, Mouse, Rat에 반응하며, IgG2a 아이소타입. 동결건조 형태로 제공되며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B5 |
| Immunogen | Full length human recombinant protein of human POLR2E produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The POLR2E gene encodes the fifth largest subunit of RNA polymerase II, responsible for synthesizing messenger RNA in eukaryotes.
This subunit is shared by other DNA-directed RNA polymerases and exists in two-fold molar excess over other subunits.
It interacts with a hepatitis virus transactivating protein, suggesting a role in transcriptional activation via this subunit.
A pseudogene is located on chromosome 11.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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