Thermo Fisher Scientific CUGBP1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive control: A549
• Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (80%), Xenopus laevis (82%), Chicken (95%), Chimpanzee (100%), Bovine (95%)
• Store as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease associated with a (CTG)n repeat expansion in the 3’-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis.
HeLa cell protein CUG-BP1 binds specifically to (CUG)8 oligonucleotides in vitro and represents the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50.
The CUG-BP/hNab50 protein is localized predominantly in the nucleus and associates with polyadenylated RNAs in vivo. In vitro RNA-binding and photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3’-UTR. Triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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