Thermo Fisher Scientific eIF3A/eIF3S10 Polyclonal Antibody
eIF3A/eIF3S10 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, IP 등 다양한 응용에 적합. 4°C에서 보관하며, 권장 유효기간은 1년. Rat과의 교차 반응 예측됨.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 860 and 910 of human translation initiation factor 3 subunit A (eukaryotic translation initiation factor 3, subunit 10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat.
Target Information
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, required for multiple steps in protein synthesis initiation. The eIF-3 complex associates with the 40S ribosome to form the 43S pre-initiation complex (PIC), facilitating mRNA recruitment and AUG recognition. It also plays roles in ribosomal subunit recycling and prevents premature subunit joining. The complex regulates translation of mRNAs involved in cell proliferation, differentiation, and apoptosis through RNA stem-loop binding mechanisms.
[UniProt References: PubMed:17581632, 25849773, 11169732, 27462815]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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