Thermo Fisher Scientific eIF3A/eIF3S10 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat, Zebrafish, and X. tropicalis

Target Information

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773).
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition, and is required for disassembly and recycling of post-termination ribosomal complexes.
The eIF-3 complex prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation and specifically targets translation of mRNAs involved in cell proliferation, differentiation, and apoptosis.
[UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.