Thermo Fisher Scientific CD336 (NKp44) Monoclonal Antibody (44.189), Functional Grade, eBioscience

Thermo Fisher Scientific CD336 (NKp44) Monoclonal Antibody (44.189), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The monoclonal antibody 44.189 reacts with human CD336, also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain in the cytoplasmic tail.
Expression and activation require association with DAP12, an ITAM-containing transmembrane accessory protein.
CD336 is expressed on IL-2-activated NK cells, gamma delta T cells, IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the intestinal epithelium, and T cells in cord blood.
It plays a role in innate immune responses against viral infection and tumor proliferation.

The 44.189 antibody facilitates CD336 crosslinking and is suitable for flow cytometric analysis and functional assays (receptor crosslinking).
Tested by flow cytometry on normal human peripheral blood cells at ≤0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test).
Optimal performance requires titration.

• Storage and Handling: Use in a sterile environment
• Filtration: 0.2 µm post-manufacturing filtered
• Purity: >90% (SDS-PAGE)
• Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

CD336 (NKp44/NCR2) is a transmembrane glycoprotein involved in NK cell activation and innate immune response. Its expression requires DAP12 association and is found in various immune cell types, including NK cells, dendritic cells, and T cell subsets. It contributes to defense against viral infection and tumor proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.