Thermo Fisher Scientific CD336 (NKp44) Monoclonal Antibody (44.189), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

44.189

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• APC
• eFluor 450
• PE
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2865959

Product Specific Information

Description: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.

The 44.189 antibody has been reported to facilitate CD336 crosslinking.

Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis and functional assays (receptor crosslinking).

Applications Tested: This 44.189 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD336 is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.