Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Functional Grade, eBioscience
E-Cadherin(CD324)을 인식하는 DECMA-1 단클론 항체로, 인간·마우스·개 세포에서 세포 간 부착 억제 기능 확인. Flow cytometry 및 기능적 차단 실험에 적합하며, 1 mg/mL 농도의 액상 형태로 제공. 4°C 보관, Wet ice 배송.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | 5 |
| Immunohistochemistry (IHC) | – | 8 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | 3 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 13 |
| Flow Cytometry (Flow) | 1 µg/test | 10 |
| Neutralization (Neu) | Assay-Dependent | 3 |
| Functional Assay (Functional) | Assay-Dependent | – |
| Inhibition Assays (IA) | – | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DECMA-1 |
| Conjugate | Functional Grade |
| Available Formats | Unconjugated, Alexa Fluor 488, Biotin, eFluor 660, PerCP-eFluor 710, Custom conjugation available |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2865950 |
| Filtration | 0.2 µm post-manufacturing filtered |
| Purity | >90% (SDS-PAGE) |
| Endotoxin Level | <0.001 ng/µg antibody (LAL assay) |
| Aggregation | <10% (HPLC) |
Product Specific Information
The monoclonal antibody DECMA-1 recognizes mouse, human, and canine CD324 (E-cadherin), also known as Epithelial cadherin or uvomorulin.
E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion, interacting with α-, β-, and γ-catenins and actinins to connect with the cytoskeleton. Expression is found in most epidermal cells, including melanocytes and keratinocytes, and is localized at intercellular boundaries of epithelial tissues, maintaining tissue integrity.
Loss of E-cadherin function is implicated in cancer progression. The protein is sensitive to trypsin; exposure should be minimized or avoided.
The DECMA-1 antibody has demonstrated functional activity in disrupting adhesion in human, mouse, and dog cells.
It is suitable for functional blocking assays and flow cytometric analysis. For flow cytometry, use ≤1 µg/test (in 100 µL final volume). Optimal staining is achieved by intracellular staining due to variable surface expression. Antibody titration is recommended for best performance.
Storage and Handling: Use in a sterile environment.
Purification: Filtered through 0.2 µm membrane post-manufacture.
Target Information
E-Cadherin (epithelial cadherin) is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
Mutations in the CDH1 gene (chromosome 16) are linked to gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-cadherin function contributes to tumor invasion and metastasis.
E-cadherin also mediates bacterial adhesion and is essential for maintaining tissue architecture and integrity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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