Thermo Fisher Scientific GSK3B Monoclonal Antibody (3D10)
GSK3B 단백질을 검출하는 Thermo Fisher의 3D10 단클론 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat 등 반응성이 있으며, 46kDa GSK3B 단백질 인식. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D10 |
| Immunogen | Purified recombinant fragment of human GSK3B expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10983646 |
Product Specific Information
MA5-15597 targets GSK3B in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Mouse, Non-human primate, and Rat samples.
The immunogen is a purified recombinant fragment of human GSK3B expressed in E. coli.
MA5-15597 detects GSK3B with a predicted molecular weight of approximately 46 kDa.
Target Information
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development, and body pattern formation. It negatively regulates glucose homeostasis and participates in energy metabolism, inflammation, ER stress, mitochondrial dysfunction, and apoptotic pathways.
GSK3B is a key component of the Wnt/β-catenin signaling pathway, mediating β-catenin degradation and inhibiting Wnt signaling. It is also involved in Hedgehog, Notch, and insulin signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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